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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in endorsing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence information is just not yet readily available.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide typically is made up of the epitope regarded from the antibody.
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This tutorial outlines additional possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
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This guideline outlines more doable optimization of mobile and immunoassay parameters to guarantee the best possible final results are...
Antibodies sure to the blocking peptide not bind to your epitope over the target protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, one can see which staining is distinct.
Focus on Information and facts Cyclin E belongs to the highly conserved cyclin family members, whose members are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and features to be a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates with the G1-S period boundary which is degraded as cells development by S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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